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验证数据展示

  • WB analysis of rat brain using 17524-1-AP

    WB analysis of rat brain using 17524-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 17524-1-AP

    WB analysis of PC-3 using 17524-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 17524-1-AP

    WB analysis of mouse brain using 17524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 17524-1-AP

    IP experiment of rat brain using 17524-1-AP

    IP result of anti-Inhibin beta A-Specific (IP:17524-1-AP, 4ug; Detection:17524-1-AP 1:500) with rat brain tissue lysate 4000ug.

Inhibin beta A-Specific Polyclonal antibody

Inhibin beta A-Specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 17524-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, PC-3 cells, rat brain tissue
Positive IP detected inrat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17524-1-AP targets Inhibin beta A-Specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibin, beta A
Synonyms Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_002192
Gene Symbol Inhibin beta A
Gene ID (NCBI) 3624
RRIDAB_2296035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibin βA (INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

实验方案

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 17524-1-APDownload protocol
IP protocol for Inhibin beta A-Specific antibody 17524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols