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验证数据展示

  • WB analysis of HepG2 using 60352-1-Ig

    WB analysis of HepG2 using 60352-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin beta A-Specific Monoclonal antibody

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60352-1-Ig

Print datasheet

CloneNo. 1F8D8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human testis tissue, L02 cells, pig liver tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name inhibin, beta A
Synonyms Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_002192
Gene Symbol Inhibin beta A
Gene ID (NCBI) 3624
RRIDAB_2881461
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

实验方案

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
IHC protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols