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INO80 Polyclonal antibody

INO80 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18810-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (4)

INO 80, hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18810-1-AP targets INO80 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INO80 homolog (S. cerevisiae)
别名 INO 80, hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1
计算分子量 1556 aa, 177 kDa
观测分子量 178 kDa
GenBank蛋白编号BC146785
基因名称 INO80
Gene ID (NCBI) 54617
RRIDAB_10598463
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity

实验方案

Product Specific Protocols
WB protocol for INO80 antibody 18810-1-APDownload protocol
IF protocol for INO80 antibody 18810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Genet

Mammalian SWI/SNF continuously restores local accessibility to chromatin.

Authors - Mario Iurlaro
  • KO Validated
mouseWB,IF,ChIP

Cell Stem Cell

INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.

Authors - Li Wang
  • KD Validated
mouseWB

Mol Cell

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Authors - Lenka Skalska
mousechIP

Mol Cell

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

Authors - Yong Xue
  • KD Validated
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB,IF

Nat Commun

Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.

Authors - Lisa Prendergast
  • KD Validated
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