验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| ChIP | See 1 publications below |
产品信息
24819-1-AP targets INO80 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | INO80 fusion protein Ag20309 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | INO80 homolog (S. cerevisiae) |
| 别名 | hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259 |
| 计算分子量 | 1556 aa, 177 kDa |
| 观测分子量 | 177 kDa, 150 kDa |
| GenBank蛋白编号 | BC146785 |
| 基因名称 | INO80 |
| Gene ID (NCBI) | 54617 |
| RRID | AB_2879741 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INO80 antibody 24819-1-AP | Download protocol |
| IHC protocol for INO80 antibody 24819-1-AP | Download protocol |
| IP protocol for INO80 antibody 24819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. | ||
Genes Dev INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma. | ||
Int J Mol Sci Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells
| ||
Sci Rep Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.
| ||
FEBS J The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.
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