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INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29353-1-AP

Print datasheet

Synonyms

INPP5E, PPI5PIV

验证数据展示

  • WB analysis using 29353-1-AP

    WB analysis using 29353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of ARPE-19 using 29353-1-AP

    IF Staining of ARPE-19 using 29353-1-AP

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of ARPE-19 using 29353-1-AP

    IF Staining of ARPE-19 using 29353-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, rat testis tissue
Positive IF detected inARPE-19 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29353-1-AP targets INPP5E in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen INPP5E fusion protein Ag30305 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Synonyms INPP5E, PPI5PIV
Calculated Molecular Weight 644 aa, 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC028032
Gene Symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

实验方案

Product Specific Protocols
WB protocol for INPP5E antibody 29353-1-APDownload protocol
IF protocol for INPP5E antibody 29353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols