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INPP5F Polyclonal antibody

INPP5F Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 27240-1-AP

Print datasheet

Synonyms

hSAC2, INPP5F, KIAA0966, MSTP007, MSTP047, MSTPO47, SAC2

验证数据展示

  • WB analysis using 27240-1-AP

    WB analysis using 27240-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 27240-1-AP

    IP experiment of MCF-7 using 27240-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:500.

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27240-1-AP targets INPP5F in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen INPP5F fusion protein Ag25687 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate-5-phosphatase F
Synonyms hSAC2, INPP5F, KIAA0966, MSTP007, MSTP047, MSTPO47, SAC2
Calculated Molecular Weight 1132 aa, 128 kDa
Observed Molecular Weight128-140 kDa
GenBank Accession NumberBC111493
Gene Symbol INPP5F
Gene ID (NCBI) 22876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2H2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5F can negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus.

实验方案

Product Specific Protocols
WB protocol for INPP5F antibody 27240-1-APDownload protocol
IP protocol for INPP5F antibody 27240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols