验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21417-1-AP targets INPP5J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | INPP5J fusion protein Ag15996 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inositol polyphosphate-5-phosphatase J |
| 别名 | INPP5, INPP5J, PIB5PA, PIPP |
| 计算分子量 | 1006 aa, 107 kDa |
| 观测分子量 | 107 kDa, 70 kDa |
| GenBank蛋白编号 | BC109288 |
| 基因名称 | INPP5J |
| Gene ID (NCBI) | 27124 |
| RRID | AB_10859784 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15735 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INPP5J(Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A) is also named as PIB5PA, PIPP and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. INPP5J is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between FRMPD4 and ARHGEF7. It can control the resting membrane potential through a specific ion-channel-receptor-ligand interaction that brings about a large conformational change, analogous to neurotransmitter activation of ion channels at synapses. (PMID:22595022). This protein has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INPP5J antibody 21417-1-AP | Download protocol |
| IHC protocol for INPP5J antibody 21417-1-AP | Download protocol |
| IF protocol for INPP5J antibody 21417-1-AP | Download protocol |
| IP protocol for INPP5J antibody 21417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun miR-508 sustains phosphoinositide signalling and promotes aggressive phenotype of oesophageal squamous cell carcinoma. | ||
Biomed Pharmacother MiR-661 contributed to cell proliferation of human ovarian cancer cells by repressing INPP5J expression. | ||
Cell Signal PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation
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