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验证数据展示

  • WB analysis of HEK-293 using 15098-1-AP

    WB analysis of HEK-293 using 15098-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15098-1-AP

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with INPP5K antibody included
  • IHC staining of human kidney using 15098-1-AP

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with INPP5K antibody included
  • IF Staining of HEK-293 using 15098-1-AP

    IF Staining of HEK-293 using 15098-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15098-1-AP (INPP5K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

INPP5K Polyclonal antibody

INPP5K Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15098-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Phosphatidylinositol-4,5-bisphosphate 5-phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate 5-phosphatase K, EC:3.1.3.86, EC:3.1.3.56

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy INPP5K Ready-To-Use IHC Kit. INPP5K primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15098-1-AP targets INPP5K in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 INPP5K fusion protein Ag7192 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase K
别名 Phosphatidylinositol-4,5-bisphosphate 5-phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate 5-phosphatase K, EC:3.1.3.86, EC:3.1.3.56
计算分子量 51 kDa
观测分子量 45-51 kDa
GenBank蛋白编号BC004362
基因名称 INPP5K
Gene ID (NCBI) 51763
RRIDAB_2126365
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INPP5K antibody 15098-1-APDownload protocol
IHC protocol for INPP5K antibody 15098-1-APDownload protocol
IF protocol for INPP5K antibody 15098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.

Authors - David Furkert
View Article
human,mouseWB,IF

Front Mol Neurosci

Knockdown of INPP5K compromises the differentiation of N2A cells

Authors - Annamaria Manzolillo
View Article
mouseWB

Exp Neurol

METTL5-mediated 18S rRNA m6A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury

Authors - Zhenpeng Li
View Article