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INPPL1 Polyclonal antibody

INPPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26678-1-AP

Print datasheet

Synonyms

INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2

验证数据展示

  • WB analysis using 26678-1-AP

    WB analysis using 26678-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26678-1-AP (INPPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26678-1-AP

    IP experiment of HeLa using 26678-1-AP

    IP result of anti-INPPL1 (IP:26678-1-AP, 4ug; Detection:26678-1-AP 1:800) with HeLa cells lysate 2520 ug.

  • IHC staining of mouse stomach using 26678-1-AP

    IHC staining of mouse stomach using 26678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPPL1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy INPPL1 Ready-To-Use IHC Kit. INPPL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26678-1-AP targets INPPL1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen INPPL1 fusion protein Ag24790 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate phosphatase-like 1
Synonyms INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2
Calculated Molecular Weight 1258 aa, 139 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC140853
Gene Symbol INPPL1
Gene ID (NCBI) 3636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15357
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INPPL1 antibody 26678-1-APDownload protocol
IHC protocol for INPPL1 antibody 26678-1-APDownload protocol
IP protocol for INPPL1 antibody 26678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols