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INSL3 Polyclonal antibody

INSL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17997-1-AP

Print datasheet

Synonyms

INSL3, Insulin like 3, insulin like 3 (Leydig cell), Ley I L, Leydig insulin like peptide, Relaxin like factor, RLF, RLNL

验证数据展示

  • WB analysis using 17997-1-AP

    WB analysis using 17997-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17997-1-AP (INSL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17997-1-AP

    IHC staining of human testis using 17997-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 17997-1-AP

    IHC staining of human testis using 17997-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17997-1-AP targets INSL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityrat
Immunogen INSL3 fusion protein Ag12455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name insulin-like 3 (Leydig cell)
Synonyms INSL3, Insulin like 3, insulin like 3 (Leydig cell), Ley I L, Leydig insulin like peptide, Relaxin like factor, RLF, RLNL
Calculated Molecular Weight 131 aa, 14 kDa
GenBank Accession NumberBC071706
Gene Symbol INSL3
Gene ID (NCBI) 3640
RRIDAB_2878479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

实验方案

Product Specific Protocols
WB protocol for INSL3 antibody 17997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Ecotoxicol Environ Saf

Identification of endocrine-disrupting chemicals targeting the genes and pathways of genital anomalies in males

Authors - Xiang Zhou
View Article