INSL3 Polyclonal antibody

INSL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17997-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

INSL3, Insulin like 3, insulin like 3 (Leydig cell), Ley I L, Leydig insulin like peptide, Relaxin like factor, RLF, RLNL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17997-1-AP targets INSL3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性rat
免疫原 INSL3 fusion protein Ag12455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like 3 (Leydig cell)
别名 INSL3, Insulin like 3, insulin like 3 (Leydig cell), Ley I L, Leydig insulin like peptide, Relaxin like factor, RLF, RLNL
计算分子量 131 aa, 14 kDa
GenBank蛋白编号BC071706
基因名称 INSL3
Gene ID (NCBI) 3640
RRIDAB_2878479
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51460
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

实验方案

Product Specific Protocols
WB protocol for INSL3 antibody 17997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Exp Mol Pathol

Transcriptomic analysis of the HPT axis in a model of oligoasthenozoospermia induced by Adenine in rats

Authors - Nan Yang
ratWB

Ecotoxicol Environ Saf

Identification of endocrine-disrupting chemicals targeting the genes and pathways of genital anomalies in males

Authors - Xiang Zhou
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