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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 27697-1-AP

    WB analysis using 27697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27697-1-AP (INSL5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

INSL5 Polyclonal antibody

INSL5 Polyclonal Antibody for WB, ELISA
Cat No. 27697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

INSL5, insulin like 5, Insulin like peptide 5, Insulin like peptide INSL5, PRO182, UNQ156

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27697-1-AP targets INSL5 in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 INSL5 fusion protein Ag26833 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like 5
别名 INSL5, insulin like 5, Insulin like peptide 5, Insulin like peptide INSL5, PRO182, UNQ156
观测分子量12-15 kDa
GenBank蛋白编号BC101646
基因名称 INSL5
Gene ID (NCBI) 10022
RRIDAB_3085985
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Q6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like peptide 5 (INSL5), also named as UNQ156/PRO182, is a member of the relaxin/insulin superfamily and presents a tertiary structure similar to that of other insulin family members. It is located outside the cell membranes and highly expressed in rectum with lower levels in uterus and ascending and descending colon (PMID: 10458910). The calculated molecular weight of INSL5 is 15 kDa. A recent report suggested an important role for Isnl5 in the regulation of insulin secretion and b-cell homeostasis. Other reports identified that colonic Isnl5 expression is reduced by the gut microbiota and energy availability. In human diseases, INSL5 has been identified recently in colonic tissue and neuroendocrine tumors, but its specific functions in tumors remain to be elucidated(PMID: 32657028).

实验方案

Product Specific Protocols
WB protocol for INSL5 antibody 27697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols