INSR Polyclonal antibody

INSR Polyclonal Antibody for WB, IP, ELISA
Cat No. 20433-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (5)

应用

WB, IP, ELISA and More (4)

CD220, HHF5, INSR, IR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20433-1-AP targets INSR in WB, IHC, IF, IP, CoIP, Dot blot, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP, Dot blot
经测试反应性 human
文献引用反应性human, mouse, rat, pig, zebrafish, fish
免疫原 INSR fusion protein Ag13922 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INSR
别名 CD220, HHF5, INSR, IR
计算分子量 1382 aa, 156 kDa
观测分子量 190 kDa, 135 kDa, 95 kDa
GenBank蛋白编号BC117172
基因名称 INSR
Gene ID (NCBI) 3643
RRIDAB_10646469
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.

实验方案

Product Specific Protocols
WB protocol for INSR antibody 20433-1-APDownload protocol
IP protocol for INSR antibody 20433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseDot blot

Environ Health Perspect

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Authors - Jacob Raber
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
humanWB

Br J Surg

MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer.

Authors - M Xiao
humanWB

FASEB J

Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.

Authors - Tong Xu
mouseWB,IHC

Sci Rep

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Authors - Shu Yang
mouseWB

J Mol Med (Berl)

Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway

Authors - Shanshan Zhang
Loading...