验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, rat testis tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15271-1-AP targets INTS10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | INTS10 fusion protein Ag7383 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrator complex subunit 10 |
| 别名 | C8orf35, INT10, integrator complex subunit 10, INTS10 |
| 计算分子量 | 82 kDa |
| 观测分子量 | 70-82 kDa |
| GenBank蛋白编号 | BC001915 |
| 基因名称 | INTS10 |
| Gene ID (NCBI) | 55174 |
| RRID | AB_2127260 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVR2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INTS10 antibody 15271-1-AP | Download protocol |
| IP protocol for INTS10 antibody 15271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII | ||
Mol Cell Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination. | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Nat Commun INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.
| ||
Nat Commun DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes. | ||
Proc Natl Acad Sci U S A Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance. |
