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INTS12 Polyclonal antibody

INTS12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16455-1-AP

Print datasheet

Synonyms

SBBI22, PHF22, Int12

验证数据展示

  • WB analysis of HepG2 using 16455-1-AP

    WB analysis of HepG2 using 16455-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 16455-1-AP

    IHC staining of human stomach using 16455-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16455-1-AP (INTS12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen INTS12 fusion protein Ag9403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrator complex subunit 12
Synonyms SBBI22, PHF22, Int12
Calculated Molecular Weight 462 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC014442
Gene Symbol INTS12
Gene ID (NCBI) 57117
RRIDAB_2127263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INTS12 antibody 16455-1-APDownload protocol
IHC protocol for INTS12 antibody 16455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
View Article
humanWB

PLoS Genet

Human mutations in integrator complex subunits link transcriptome integrity to brain development.

Authors - Renske Oegema
View Article
humanWB

Cell Rep

A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder

Authors - Hanzhe Kuang
View Article