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验证数据展示

  • WB analysis using 67060-1-Ig

    WB analysis using 67060-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis of HEK-293 using 67060-1-Ig

    WB analysis of HEK-293 using 67060-1-Ig

    WB result of INTS3 antibody (67060-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.

  • IF Staining of MCF-7 using 67060-1-Ig

    IF Staining of MCF-7 using 67060-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INTS3 antibody (67060-1-Ig, Clone: 2G11B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

INTS3 Monoclonal antibody

INTS3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67060-1-Ig

产品说明书

CloneNo. 2G11B7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

2G11B7, C1orf193, C1orf60, Int3, Integrator complex subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, 4T1 cells, MCF-7 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy INTS3 Ready-To-Use IHC Kit. INTS3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67060-1-Ig targets INTS3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 INTS3 fusion protein Ag9917 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 integrator complex subunit 3
别名 2G11B7, C1orf193, C1orf60, Int3, Integrator complex subunit 3
计算分子量 118 kDa
观测分子量 118 kDa
GenBank蛋白编号BC025254
基因名称 INTS3
Gene ID (NCBI) 65123
RRIDAB_2882370
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ68E01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INTS3 antibody 67060-1-IgDownload protocol
IF protocol for INTS3 antibody 67060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols