验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, K-562 cells, L02 cells, SH-SY5Y cells, mouse skeletal muscle tissue, HepG2 cells, Raji cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10585-1-AP targets INVS in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, FC, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | INVS fusion protein Ag0934 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inversin |
| 别名 | INV, inversin, INVS, KIAA0573, Nephrocystin 2 |
| 计算分子量 | 118 kDa, 100 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC006370 |
| 基因名称 | INVS |
| Gene ID (NCBI) | 27130 |
| RRID | AB_2233902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y283 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INVS antibody 10585-1-AP | Download protocol |
| IHC protocol for INVS antibody 10585-1-AP | Download protocol |
| IF protocol for INVS antibody 10585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Cell type-specific regulation of ciliary transition zone assembly in vertebrates.
| ||
Proc Natl Acad Sci U S A A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia. | ||
Endocrinology PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.
| ||
Virchows Arch Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats. | ||
Am J Med Genet A Early presentation of cystic kidneys in a family with a homozygous INVS mutation. | ||
