验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, MCF-7 cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30161-1-AP targets IPMK in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | IPMK fusion protein Ag32377 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | inositol polyphosphate multikinase |
Synonyms | IMPK, IPMK |
Observed Molecular Weight | 42-47 kDa |
GenBank Accession Number | BC065709 |
Gene Symbol | IPMK |
Gene ID (NCBI) | 253430 |
RRID | AB_3086252 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8NFU5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).
实验方案
Product Specific Protocols | |
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WB protocol for IPMK antibody 30161-1-AP | Download protocol |
IF protocol for IPMK antibody 30161-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |