验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14403-1-AP targets IPO11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | IPO11 fusion protein Ag5659 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | importin 11 |
| 别名 | RanBP11, Ran-binding protein 11, Ran binding protein 11, Importin-11, importin 11 |
| 计算分子量 | 113 kDa |
| 观测分子量 | 112 kDa |
| GenBank蛋白编号 | BC033776 |
| 基因名称 | IPO11 |
| Gene ID (NCBI) | 51194 |
| RRID | AB_2296214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UI26 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. Importin-11, is a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IPO11 antibody 14403-1-AP | Download protocol |
| IHC protocol for IPO11 antibody 14403-1-AP | Download protocol |
| IF protocol for IPO11 antibody 14403-1-AP | Download protocol |
| IP protocol for IPO11 antibody 14403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. | ||
Urol Oncol Importin-11 overexpression promotes the migration, invasion, and progression of bladder cancer associated with the deregulation of CDKN1A and THBS1.
| ||
Int J Med Sci Importin-11 is Essential for Normal Embryonic Development in Mice.
| ||
Oncotarget Whole-exome sequencing of muscle-invasive bladder cancer identifies recurrent copy number variation in IPO11 and prognostic significance of importin-11 overexpression on poor survival. |