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  • KD/KO Validated

IPO13 Polyclonal antibody

IPO13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11696-2-AP

Print datasheet

Synonyms

KIAA0724, Karyopherin-13, Karyopherin 13, Kap13, Importin-13



经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue, Neura-2a cells, mouse lung
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11696-2-AP targets IPO13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen IPO13 fusion protein Ag2317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name importin 13
Synonyms KIAA0724, Karyopherin-13, Karyopherin 13, Kap13, Importin-13
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC008194
Gene Symbol IPO13
Gene ID (NCBI) 9670
RRIDAB_2127672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94829
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IPO13 antibody 11696-2-APDownload protocol
IHC protocol for IPO13 antibody 11696-2-APDownload protocol
IF protocol for IPO13 antibody 11696-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Nat Commun

Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.

Authors - K A Gajewska
  • KD Validated
humanWB,IHC,IF

Stem Cells

Importin 13 serves as a potential marker for corneal epithelial progenitor cells.

Authors - Wang Hua H
mouseWB

Data Brief

Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress.

Authors - Katarzyna A Gajewska
  • KO Validated
humanWB

Cells

The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis

Authors - Katarzyna A Gajewska
  • KD Validated