验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11679-1-AP targets IPO4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | IPO4 fusion protein Ag2281 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | importin 4 |
| 别名 | Ran-binding protein 4, Importin-4b, Importin-4, Importin 4b, importin 4 |
| 计算分子量 | 1081 aa, 118 kDa |
| 观测分子量 | 118 kDa |
| GenBank蛋白编号 | BC003690 |
| 基因名称 | IPO4 |
| Gene ID (NCBI) | 79711 |
| RRID | AB_2127677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEX9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IHC protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IF protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IP protocol for IPO4 antibody 11679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development. | ||
Proc Natl Acad Sci U S A CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage. | ||
Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. | ||
Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. | ||
PLoS One An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. | ||
