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IPO4 Monoclonal antibody, PBS Only

IPO4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F3H2

Cat no : 67549-1-PBS

Print datasheet

Synonyms

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

验证数据展示

  • WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

    WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

    IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

产品信息

67549-1-PBS targets IPO4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen IPO4 fusion protein Ag2281 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name importin 4
Synonyms FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4
Calculated Molecular Weight 1081 aa, 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003690
Gene Symbol IPO4
Gene ID (NCBI) 79711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TEX9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.