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验证数据展示

  • WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

    WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

    IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

IPO4 Monoclonal antibody, PBS Only

IPO4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67549-1-PBS

产品说明书

CloneNo. 2F3H2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67549-1-PBS targets IPO4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 IPO4 fusion protein Ag2281 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 importin 4
别名 FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4
计算分子量 1081 aa, 118 kDa
观测分子量 120 kDa
GenBank蛋白编号BC003690
基因名称 IPO4
Gene ID (NCBI) 79711
RRIDAB_2882764
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TEX9
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.