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IPO5 Monoclonal antibody, PBS Only

IPO5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2B9

Cat no : 67962-1-PBS

Print datasheet

Synonyms

DKFZp686O1576, FLJ43041, IMB3, Imp5, importin 5, Importin subunit beta 3, IPO5, Karyopherin beta 3, KPNB3, Ran binding protein 5, RANBP5

验证数据展示

  • WB analysis using 67962-1-Ig (same clone as 67962-1-PBS)

    WB analysis using 67962-1-Ig (same clone as 67962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67962-1-Ig (IPO5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67962-1-Ig (same clone as 67962-1-PBS)

    IHC staining of mouse testis using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67962-1-Ig (IPO5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67962-1-Ig (same clone as 67962-1-PBS)

    IF Staining of A431 using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IPO5 antibody (67962-1-Ig, Clone: 1E2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

产品信息

67962-1-PBS targets IPO5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen IPO5 fusion protein Ag30955 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name importin 5
Synonyms DKFZp686O1576, FLJ43041, IMB3, Imp5, importin 5, Importin subunit beta 3, IPO5, Karyopherin beta 3, KPNB3, Ran binding protein 5, RANBP5
Observed Molecular Weight124 kDa
GenBank Accession NumberBC001497
Gene Symbol IPO5
Gene ID (NCBI) 3843
RRIDAB_2918713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00410
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.