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CoraLite® Plus 488-conjugated IPO5 Monoclonal antibody

IPO5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2B9

Cat no : CL488-67962

Print datasheet

Synonyms

IPO5, Karyopherin beta 3, Karyopherin beta-3, KPNB3, Ran binding protein 5, Ran-binding protein 5, RANBP5, 1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3, Importin subunit beta-3, Importin-5

验证数据展示

  • FC experiment of A431 using CL488-67962

    FC experiment of A431 using CL488-67962

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IPO5 (CL488-67962, Clone:1E2B9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67962-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67962 targets IPO5 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen IPO5 fusion protein Ag30955 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name importin 5
Synonyms IPO5, Karyopherin beta 3, Karyopherin beta-3, KPNB3, Ran binding protein 5, Ran-binding protein 5, RANBP5, 1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3, Importin subunit beta-3, Importin-5
Observed Molecular Weight124 kDa
GenBank Accession NumberBC001497
Gene Symbol IPO5
Gene ID (NCBI) 3843
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00410
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IPO5 antibody CL488-67962Download protocol
Standard Protocols
Click here to view our Standard Protocols