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  • Featured Product
  • KD/KO Validated

IPPK Polyclonal antibody

IPPK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12603-1-AP

Print datasheet

Synonyms

C9orf12, Ins(1,3,4,5,6)P5 2 kinase, InsP5 2 kinase, INSP5K2, IP5K, IPK1, IPK1 homolog, IPPK, KIAA0699

验证数据展示

  • WB analysis of Jurkat using 12603-1-AP

    WB analysis of Jurkat using 12603-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12603-1-AP (IPPK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12603-1-AP

    IHC staining of human gliomas using 12603-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12603-1-AP (IPPK antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12603-1-AP targets IPPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen IPPK fusion protein Ag3278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Synonyms C9orf12, Ins(1,3,4,5,6)P5 2 kinase, InsP5 2 kinase, INSP5K2, IP5K, IPK1, IPK1 homolog, IPPK, KIAA0699
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC026154
Gene Symbol IPPK
Gene ID (NCBI) 64768
RRIDAB_2127848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8X2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IPPK antibody 12603-1-APDownload protocol
IHC protocol for IPPK antibody 12603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

Authors - Paul C Scherer
  • KD Validated
View Article