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IQCK Polyclonal antibody

IQCK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25740-1-AP

Print datasheet

Synonyms

IQ domain containing protein K, IQ motif containing K, IQCK

验证数据展示

  • WB analysis of mouse testis using 25740-1-AP

    WB analysis of mouse testis using 25740-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25740-1-AP (IQCK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25740-1-AP

    IHC staining of human colon cancer using 25740-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 25740-1-AP

    IHC staining of human colon cancer using 25740-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of COLO 320 using 25740-1-AP

    IF Staining of COLO 320 using 25740-1-AP

    Immunofluorescent analysis of COLO 320 cells using 25740-1-AP (IQCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25740-1-AP is IQCK Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen IQCK fusion protein Ag22680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IQ motif containing K
Synonyms IQ domain containing protein K, IQ motif containing K, IQCK
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC034823
Gene Symbol IQCK
Gene ID (NCBI) 124152
RRIDAB_2880220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0W5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IQCK antibody 25740-1-APDownload protocol
IHC protocol for IQCK antibody 25740-1-APDownload protocol
IF protocol for IQCK antibody 25740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,WB,IF

Front Cell Neurosci

Novel Alzheimer risk factor IQ motif containing protein K is abundantly expressed in the brain and is markedly increased in patients with Alzheimer's disease.

Authors - Hongjie Wang
View Article