IQGAP1 Monoclonal antibody

IQGAP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68588-1-Ig

产品说明书

CloneNo. 2E11A2

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, ELISA

Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68588-1-Ig targets IQGAP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 IQGAP1 fusion protein Ag19321 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 IQ motif containing GTPase activating protein 1
别名 Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2
计算分子量 189 kDa
观测分子量 190-195 kDa
GenBank蛋白编号BC151834
基因名称 IQGAP1
Gene ID (NCBI) 8826
ENSEMBL Gene IDENSG00000140575
RRIDAB_3085287
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46940
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

实验方案

Product Specific Protocols
WB protocol for IQGAP1 antibody 68588-1-IgDownload protocol
IHC protocol for IQGAP1 antibody 68588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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