验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68588-1-Ig targets IQGAP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, rat |
免疫原 | IQGAP1 fusion protein Ag19321 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | IQ motif containing GTPase activating protein 1 |
别名 | Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2 |
计算分子量 | 189 kDa |
观测分子量 | 190-195 kDa |
GenBank蛋白编号 | BC151834 |
基因名称 | IQGAP1 |
Gene ID (NCBI) | 8826 |
ENSEMBL Gene ID | ENSG00000140575 |
RRID | AB_3085287 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P46940 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.
实验方案
Product Specific Protocols | |
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WB protocol for IQGAP1 antibody 68588-1-Ig | Download protocol |
IHC protocol for IQGAP1 antibody 68588-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |