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验证数据展示

  • IP experiment of mouse testis using 25605-1-AP

    IP experiment of mouse testis using 25605-1-AP

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

  • IP experiment of mouse spleen using 25605-1-AP

    IP experiment of mouse spleen using 25605-1-AP

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

  • IHC staining of mouse testis using 25605-1-AP

    IHC staining of mouse testis using 25605-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25605-1-AP

    IHC staining of mouse testis using 25605-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IQUB Polyclonal antibody

IQUB Polyclonal Antibody for IHC, IP, ELISA
Cat No. 25605-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IP, ELISA

别名

IQ motif and ubiquitin-like domain-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inmouse spleen tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25605-1-AP targets IQUB in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 IQUB fusion protein Ag19507 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IQ motif and ubiquitin domain containing
别名 IQ motif and ubiquitin-like domain-containing protein
计算分子量 791 aa, 93 kDa
观测分子量 70 kDa
GenBank蛋白编号BC128181
基因名称 IQUB
Gene ID (NCBI) 154865
RRIDAB_3085807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NA54
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).

实验方案

Product Specific Protocols
IHC protocol for IQUB antibody 25605-1-APDownload protocol
IP protocol for IQUB antibody 25605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols