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验证数据展示

  • WB analysis using 28766-1-AP

    WB analysis using 28766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 28766-1-AP

    IHC staining of mouse colon using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included
  • IHC staining of rat small intestine using 28766-1-AP

    IHC staining of rat small intestine using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included

IRAK3 Polyclonal antibody

IRAK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

IRAK M, IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRAK3 Ready-To-Use IHC Kit. IRAK3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28766-1-AP targets IRAK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IRAK3 fusion protein Ag30309 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 3
别名 IRAK M, IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M
计算分子量 68 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC057800
基因名称 IRAK3
Gene ID (NCBI) 11213
RRIDAB_2881211
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y616
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IRAK3 antibody 28766-1-APDownload protocol
IHC protocol for IRAK3 antibody 28766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols