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验证数据展示

  • WB analysis using 29976-1-AP

    WB analysis using 29976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 29976-1-AP

    IP experiment of mouse liver using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

  • IP experiment of A549 using 29976-1-AP

    IP experiment of A549 using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug.

  • IHC staining of human colon cancer using 29976-1-AP

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IREB2 antibody included
  • IHC staining of human colon cancer using 29976-1-AP

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IREB2 antibody included
  • IF Staining of A549 using 29976-1-AP

    IF Staining of A549 using 29976-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IREB2 Polyclonal antibody

IREB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 29976-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

IRP2, Iron-responsive element-binding protein 2, IREBP2, IRE-BP 2, IREB 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inA549 cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy IREB2 Ready-To-Use IHC Kit. IREB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29976-1-AP targets IREB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 IREB2 fusion protein Ag32233 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 iron-responsive element binding protein 2
别名 IRP2, Iron-responsive element-binding protein 2, IREBP2, IRE-BP 2, IREB 2
计算分子量 105 kDa
观测分子量105 kDa
GenBank蛋白编号BC017880
基因名称 IREB2
Gene ID (NCBI) 3658
RRIDAB_3086201
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48200
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

实验方案

Product Specific Protocols
WB protocol for IREB2 antibody 29976-1-APDownload protocol
IHC protocol for IREB2 antibody 29976-1-APDownload protocol
IF protocol for IREB2 antibody 29976-1-APDownload protocol
IP protocol for IREB2 antibody 29976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols