验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy IRF2BP2 Ready-To-Use IHC Kit. IRF2BP2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18847-1-AP targets IRF2BP2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interferon regulatory factor 2 binding protein 2 |
| 别名 | IRF2BP 2, IRF 2BP2, Interferon regulatory factor 2-binding protein 2 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | NM_182972 |
| 基因名称 | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_10598163 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z5L9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IHC protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IF protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IP protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.
| ||
Hepatology The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop. | ||
Cell Death Dis SPOP point mutations regulate substrate preference and affect its function | ||
EMBO Rep Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling. | ||
Hypertension Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).
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