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Conjugate
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IRGQ Polyclonal antibody

IRGQ Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23239-1-AP

Print datasheet

Synonyms

FKSG27, IRGQ, IRGQ1

验证数据展示

  • WB analysis of A2780 using 23239-1-AP

    WB analysis of A2780 using 23239-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 23239-1-AP (IRGQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 23239-1-AP

    IHC staining of human skeletal muscle using 23239-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23239-1-AP (IRGQ Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 23239-1-AP

    IHC staining of human skeletal muscle using 23239-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23239-1-AP (IRGQ Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA2780 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23239-1-AP is IRGQ Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen IRGQ fusion protein Ag19607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunity-related GTPase family, Q
Synonyms FKSG27, IRGQ, IRGQ1
Calculated Molecular Weight 623 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC137020
Gene Symbol IRGQ
Gene ID (NCBI) 126298
RRIDAB_2879238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WZA9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IRGQ antibody 23239-1-APDownload protocol
IHC protocol for IRGQ antibody 23239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols