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IRX6 Polyclonal antibody

IRX6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24440-1-AP

Print datasheet

Synonyms

IRXB3, IRX7, IRX 3, Iroquois-class homeodomain protein IRX-6, iroquois homeobox 6

验证数据展示

  • WB analysis using 24440-1-AP

    WB analysis using 24440-1-AP

    Mouse Heart tissue lysates were subjected to SDS PAGE followed by western blot with 24440-1-AP (IRX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24440-1-AP

    IHC staining of human colon cancer using 24440-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24440-1-AP

    IHC staining of human colon cancer using 24440-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24440-1-AP targets IRX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IRX6 fusion protein Ag19623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name iroquois homeobox 6
Synonyms IRXB3, IRX7, IRX 3, Iroquois-class homeodomain protein IRX-6, iroquois homeobox 6
Calculated Molecular Weight 446 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC136573
Gene Symbol IRX6
Gene ID (NCBI) 79190
RRIDAB_2918082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78412
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRX6 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.

实验方案

Product Specific Protocols
WB protocol for IRX6 antibody 24440-1-APDownload protocol
IHC protocol for IRX6 antibody 24440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols