验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, Raji cells, A549 cells, HeLa cells, COLO 320 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14812-1-AP targets ISCU in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ISCU fusion protein Ag6562 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | iron-sulfur cluster scaffold homolog (E. coli) |
| 别名 | NIFU, ISU2, ISCU2, Iron-sulfur cluster assembly enzyme ISCU, hnifU |
| 计算分子量 | 18 kDa |
| 观测分子量 | 14-15 kDa, 18-19 kDa |
| GenBank蛋白编号 | BC061903 |
| 基因名称 | ISCU |
| Gene ID (NCBI) | 23479 |
| RRID | AB_2280362 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1K1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins.
Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ISCU antibody 14812-1-AP | Download protocol |
| IHC protocol for ISCU antibody 14812-1-AP | Download protocol |
| IF protocol for ISCU antibody 14812-1-AP | Download protocol |
| IP protocol for ISCU antibody 14812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.
| ||
Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. | ||
Cell Metab Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery. | ||
Sci Adv Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation. | ||
Transl Res Skeletal Muscle MiR-210 Expression is Associated with Mitochondrial Function in Peripheral Artery Disease Patients. | ||
Redox Biol Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis. |
