ISG15 Monoclonal antibody

ISG15 Monoclonal Antibody for WB, ELISA
Cat No. 68408-1-Ig

产品说明书

CloneNo. 3G9A2

宿主/亚型

Mouse / IgG2a

种属反应性

Human and More (1)

应用

WB, ELISA and More (1)

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMDA-MB-231 cells, LNCaP cells, A431 cells, HeLa cells, HCT 116 cells, HeLa cells, DU 145 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

68408-1-Ig targets ISG15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用IHC
经测试反应性 Human
文献引用反应性human
免疫原 ISG15 fusion protein Ag8953 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ISG15 ubiquitin-like modifier
别名 G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP
计算分子量 165 aa, 18 kDa
观测分子量 13-17 kDa
GenBank蛋白编号BC009507
基因名称 ISG15
Gene ID (NCBI) 9636
RRIDAB_3085125
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05161
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

实验方案

Product Specific Protocols
WB protocol for ISG15 antibody 68408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Exp Mol Pathol

The relationship between tumor immunity and the cGAS-STING pathway in breast cancer: An immunohistochemical study

Authors - Haruto Nishida
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