验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Daudi cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ISG20 Ready-To-Use IHC Kit. ISG20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22097-1-AP targets ISG20 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine, monkey |
| 免疫原 | ISG20 fusion protein Ag17303 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interferon stimulated exonuclease gene 20kDa |
| 别名 | HEM45, ISG20 |
| 计算分子量 | 181 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC007922 |
| 基因名称 | ISG20 |
| Gene ID (NCBI) | 3669 |
| RRID | AB_2878994 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AZ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ISG20 antibody 22097-1-AP | Download protocol |
| IHC protocol for ISG20 antibody 22097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep Placenta-derived interferon-stimulated gene 20 controls ZIKA virus infection.
| ||
J Virol Lnc-ISG20 inhibits influenza A virus replication by enhancing ISG20 expression.
| ||
Cancer Cell Int Identification and validation of a novel glycolysis-related gene signature for predicting the prognosis in ovarian cancer. | ||
Ann Transl Med Identification of key biomarkers and immune infiltration in renal interstitial fibrosis. | ||
Oncoimmunology Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma. |
