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验证数据展示

  • WB analysis using 24306-1-AP

    WB analysis using 24306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24306-1-AP (ISLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 24306-1-AP

    IF Staining of A549 using 24306-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ISLR antibody (24306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ISLR Polyclonal antibody

ISLR Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24306-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, rat thyroid gland tissue, mouse retina tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24306-1-AP targets ISLR in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 ISLR fusion protein Ag19446 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin superfamily containing leucine-rich repeat
别名 HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215
计算分子量 428 aa, 46 kDa
观测分子量44-46 kDa
GenBank蛋白编号BC022478
基因名称 ISLR
Gene ID (NCBI) 3671
RRIDAB_3085729
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14498
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

实验方案

Product Specific Protocols
WB protocol for ISLR antibody 24306-1-APDownload protocol
IF protocol for ISLR antibody 24306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols