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ISOC1 Polyclonal antibody

ISOC1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25844-1-AP

Print datasheet

Synonyms

CGI 111, ISOC1

验证数据展示

  • WB analysis using 25844-1-AP

    WB analysis using 25844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25844-1-AP

    IP experiment of mouse liver using 25844-1-AP

    IP result of anti-ISOC1 (IP:25844-1-AP, 4ug; Detection:25844-1-AP 1:300) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHT-29 cells, mouse liver tissue, HepG2 cells, SH-SY5Y cells, mouse embryo tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25844-1-AP targets ISOC1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ISOC1 fusion protein Ag22611 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isochorismatase domain containing 1
Synonyms CGI 111, ISOC1
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014105
Gene Symbol ISOC1
Gene ID (NCBI) 51015
RRIDAB_2918095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CN7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ISOC1 antibody 25844-1-APDownload protocol
IP protocol for ISOC1 antibody 25844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols