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验证数据展示

  • WB analysis using 27082-1-AP

    WB analysis using 27082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27082-1-AP (ISOC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27082-1-AP

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27082-1-AP

    IHC staining of human kidney using 27082-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27082-1-AP (ISOC2 Antibody) at dilution of 1:200 (under 40x lens).

ISOC2 Polyclonal antibody

ISOC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27082-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ISOC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27082-1-AP targets ISOC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ISOC2 fusion protein Ag23936 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isochorismatase domain containing 2
别名 ISOC2
观测分子量 28 kDa
GenBank蛋白编号BC017344
基因名称 ISOC2
Gene ID (NCBI) 79763
RRIDAB_2880748
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AB3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ISOC2 antibody 27082-1-APDownload protocol
IHC protocol for ISOC2 antibody 27082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols