验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, U-251 cells, NIH/3T3 cells, K-562 cells, Hela cells, Raji cells |
| Positive IP detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ITCH Ready-To-Use IHC Kit. ITCH primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20920-1-AP targets ITCH in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ITCH fusion protein Ag15120 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | itchy E3 ubiquitin protein ligase homolog (mouse) |
| 别名 | HECT-type E3 ubiquitin transferase Itchy homolog, EC:2.3.2.26, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, AIP4 |
| 计算分子量 | 903 aa, 103 kDa |
| 观测分子量 | 103 kDa, 70 kDa |
| GenBank蛋白编号 | BC011571 |
| 基因名称 | ITCH |
| Gene ID (NCBI) | 83737 |
| RRID | AB_2878765 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96J02 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITCH is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. It is involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. ITCH can be cleaved by caspase-3, -6, and -7 on residue Asp240 and form a 65 kDa cleaved form (PMID: 19541840).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITCH antibody 20920-1-AP | Download protocol |
| IHC protocol for ITCH antibody 20920-1-AP | Download protocol |
| IP protocol for ITCH antibody 20920-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome.
| ||
Dev Cell ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
| ||
Theranostics ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. | ||
Clin Transl Med GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.
| ||
Front Oncol Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.
| ||
J Mol Med (Berl) Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology. |
