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Integrin Alpha 4 Polyclonal antibody

Integrin Alpha 4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19676-1-AP

Print datasheet

Synonyms

CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha



经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19676-1-AP targets Integrin Alpha 4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Synonyms CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberNM_000885
Gene Symbol Integrin alpha 4
Gene ID (NCBI) 3676
RRIDAB_10640907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13612
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha 4 antibody 19676-1-APDownload protocol
IHC protocol for Integrin Alpha 4 antibody 19676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease.

Authors - Masayoshi Ohkuro
mouseWB

J Exp Med

EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.

Authors - Xixi Tao
humanWB

Int J Biol Macromol

Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis

Authors - Feng Jiang
human,mouseWB

Int J Mol Sci

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

Authors - Vincent Yi-Fong Su
humanWB

Int J Mol Sci

Sesquiterpene Lactone Deoxyelephantopin Isolated from Elephantopus scaber and Its Derivative DETD-35 Suppress BRAFV600E Mutant Melanoma Lung Metastasis in Mice.

Authors - Biljana Cvetanova
humanWB

J Neuroinflammation

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Authors - Ceshu Gao