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Integrin alpha-5 Polyclonal antibody

Integrin alpha-5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10569-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, monkey and More (1)

应用

WB, IHC, ELISA and More (1)

ITGA5, Integrin alpha-F, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10569-1-AP targets Integrin alpha-5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, monkey
文献引用反应性human, mouse, rat
免疫原 Integrin alpha-5 fusion protein Ag0860 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
别名 ITGA5, Integrin alpha-F, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA
计算分子量 115 kDa
观测分子量 135-150 kDa
GenBank蛋白编号BC008786
基因名称 Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_2130060
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08648
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 10569-1-APDownload protocol
IHC protocol for Integrin alpha-5 antibody 10569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Biomaterials

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Authors - Yonglin Yu
mouseWB

Int J Mol Sci

Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway

Authors - Zanyan Zhao
humanWB,IF

Front Cell Dev Biol

Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.

Authors - Chengyun Pan
humanWB

Oncotarget

Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.

Authors - Huimin Wang

Sci Rep

Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.

Authors - Mayra Rachele Zarone
humanIF

Front Oncol

ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas.

Authors - Shuyu Li
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