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- KD/KO Validated
Integrin Alpha 5 Polyclonal antibody
Integrin Alpha 5 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, human placenta tissue, U2OS cells |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
The immunogen of 27224-1-AP is Integrin Alpha 5 Fusion Protein expressed in E. coli.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Integrin Alpha 5 fusion protein Ag26098 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
Synonyms | CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A |
Calculated Molecular Weight | 115 kDa |
Observed Molecular Weight | 135-150 kDa |
GenBank Accession Number | NM_002205 |
Gene Symbol | Integrin alpha 5 |
Gene ID (NCBI) | 3678 |
RRID | AB_2880807 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
IHC protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
IP protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Clin Transl Hepatol A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes | ||
Mol Cancer ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner | ||
Biomater Res Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment
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