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验证数据展示

  • WB analysis of PC-3 using 12300-1-AP

    WB analysis of PC-3 using 12300-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse thymus using 12300-1-AP

    IP experiment of mouse thymus using 12300-1-AP

    IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

  • IHC staining of human heart using 12300-1-AP

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 12300-1-AP

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-3 using 12300-1-AP

    IF Staining of PC-3 using 12300-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ITGB1BP1 Polyclonal antibody

ITGB1BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12300-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse thymus tissue, human brain tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ITGB1BP1 fusion protein Ag2945 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin beta 1 binding protein 1
别名 Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1
计算分子量 200 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC012264
基因名称 ITGB1BP1
Gene ID (NCBI) 9270
RRIDAB_10640896
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14713
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.

实验方案

Product Specific Protocols
WB protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IHC protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IF protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IP protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols