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Centromere protein R Polyclonal antibody
Centromere protein R Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ITGB3BP Ready-To-Use IHC Kit. ITGB3BP primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10743-1-AP targets Centromere protein R in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Centromere protein R fusion protein Ag1204 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | integrin beta 3 binding protein (beta3-endonexin) |
| Synonyms | ITGB3BP, HSU37139, CENP-R, CENPR, CENP R |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC009929 |
| Gene Symbol | ITGB3BP |
| Gene ID (NCBI) | 23421 |
| RRID | AB_2128916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGB3BP, also named as CENPR and NRIF3; is a transcription coregulator that can have both coactivator and corepressor functions. ITGB3BP acts as a coactivator for estrogen receptor alpha. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. ITGB3BP induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP has five isoforms with MW 7 kDa, 13 kDa and 19-25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| IF protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| IP protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Identification of a novel pathway that selectively modulates apoptosis of breast cancer cells. | ||
J Mol Cell Biol Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation
| ||
Oncogene Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation. |
