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验证数据展示

  • WB analysis of BxPC-3 using 25277-1-AP

    WB analysis of BxPC-3 using 25277-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25277-1-AP

    IHC staining of human breast cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IHC staining of human breast cancer using 25277-1-AP

    IHC staining of human breast cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IF Staining of A431 using 25277-1-AP

    IF Staining of A431 using 25277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin Beta 4 antibody (25277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Integrin Beta 4 Polyclonal antibody

Integrin Beta 4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25277-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, mouse pancreas tissue, rat skin tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ITGB4 Ready-To-Use IHC Kit. ITGB4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25277-1-AP targets Integrin Beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Integrin Beta 4 fusion protein Ag16460 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, beta 4
别名 CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4
计算分子量 1752 aa, 195 kDa
观测分子量 200 kDa
GenBank蛋白编号BC126411
基因名称 Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_2880002
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

实验方案

Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
IHC protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
IF protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

A mutation found in esophageal cancer alters integrin β4 mRNA splicing.

Authors - Gabriel T Kelly
View Article
mouseIF

J Oral Biosci

Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion

Authors - Shoma Yamamori
View Article