验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, human lung tissue, K-562 cells, mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11328-1-AP targets Integrin Beta 7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Integrin Beta 7 fusion protein Ag1871 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta 7 |
| 别名 | Integrin beta 7, integrin, beta 7, ITGB7 |
| 计算分子量 | 798 aa, 87 kDa |
| 观测分子量 | 87-100 kDa |
| GenBank蛋白编号 | BC015916 |
| 基因名称 | Integrin beta 7 |
| Gene ID (NCBI) | 3695 |
| RRID | AB_2249380 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26010 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGB7 (integrin, beta7) is a single-pass type I membrane protein that belongs to the integrin beta chain family. The integrins are a large family of cell-surface glycoprotein receptors that play key roles in leucocyte adhesion, signalling, proliferation and migration by binding as heterodimers to specific ligands. The beta7 integrin associates with alpha4 integrin to form a heterodimer, alpha4/beta7, which interacts with MADCAM1, VCAM, fibronectin, and may also interact with HIV-1 gp120. The beta7 integrin can also form a heterodimer with alphaE integrin, and the dimer, alphaE/beta7 integrin, is a receptor for E-cadherin. ITGB7 is expressed in a variety of leukocyte lines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 7 antibody 11328-1-AP | Download protocol |
| IHC protocol for Integrin Beta 7 antibody 11328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Reprod Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis. | ||
J Biol Chem Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on DSS-induced murine colitis. | ||
Cell Commun Signal Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis. | ||
Cell Mol Gastroenterol Hepatol Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation | ||
Arthritis Res Ther Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression | ||
J Ethnopharmacol Dan'e fukang decoction reduces hemorrhage in a rat model of mifepristone induced incomplete abortion and may correlate with cell adhesion molecule signaling interference |
