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ITK Monoclonal antibody, PBS Only

ITK Monoclonal Antibody for WB,Indirect ELISA,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

1F9D8

Cat No : 67703-1-PBS

Print datasheet

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

验证数据展示

  • WB analysis of PBMC using 67703-1-Ig (same clone as 67703-1-PBS)

    WB analysis of PBMC using 67703-1-Ig (same clone as 67703-1-PBS)

    PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    WB analysis of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    FC experiment of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITK (67703-1-Ig, Clone:1F9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    IF Staining of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

产品信息

67703-1-PBS targets ITK in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IF,FC Application Description
Tested Reactivity Human
Immunogen ITK fusion protein Ag17087 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IL2-inducible T-cell kinase
Synonyms EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08881
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).