验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human heart tissue, human lung cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
16134-1-AP targets ITPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ITPA fusion protein Ag9128 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inosine triphosphatase (nucleoside triphosphate pyrophosphatase) |
| 别名 | Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9 |
| 计算分子量 | 194 aa, 21 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC010138 |
| 基因名称 | ITPA |
| Gene ID (NCBI) | 3704 |
| RRID | AB_2129821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BY32 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPA antibody 16134-1-AP | Download protocol |
| IHC protocol for ITPA antibody 16134-1-AP | Download protocol |
| IF protocol for ITPA antibody 16134-1-AP | Download protocol |
| IP protocol for ITPA antibody 16134-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase. |
