ITPA Polyclonal antibody

ITPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16134-1-AP

Print datasheet

Synonyms

Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9



经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16134-1-AP targets ITPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ITPA fusion protein Ag9128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Synonyms Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9
Calculated Molecular Weight 194 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC010138
Gene Symbol ITPA
Gene ID (NCBI) 3704
RRIDAB_2129821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

实验方案

Product Specific Protocols
WB protocol for ITPA antibody 16134-1-APDownload protocol
IHC protocol for ITPA antibody 16134-1-APDownload protocol
IF protocol for ITPA antibody 16134-1-APDownload protocol
IP protocol for ITPA antibody 16134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochimie

Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.

Authors - Hill Amanda A
humanIHC

Oncotarget

A four-gene signature predicts survival in clear-cell renal-cell carcinoma.

Authors - Jun Dai
humanWB

Biochim Biophys Acta

Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.

Authors - Herting Greg G