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ITPK1 Polyclonal antibody

ITPK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10568-1-AP

Print datasheet

Synonyms

Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1

验证数据展示

  • IHC staining of human brain using 10568-1-AP

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 10568-1-AP

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 10568-1-AP

    WB analysis of human brain using 10568-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, mouse eye tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10568-1-AP targets ITPK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ITPK1 fusion protein Ag0834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol 1,3,4-triphosphate 5/6 kinase
Synonyms Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC007428
Gene Symbol ITPK1
Gene ID (NCBI) 3705
RRIDAB_2129832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITPK1 antibody 10568-1-APDownload protocol
IHC protocol for ITPK1 antibody 10568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols