验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3200 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14270-1-AP targets ITPKA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ITPKA fusion protein Ag5588 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inositol 1,4,5-trisphosphate 3-kinase A |
| 别名 | IP3K A, IP3 3-kinase A, InsP 3-kinase A, Inositol-trisphosphate 3-kinase A, Inositol 1,4,5-trisphosphate 3-kinase A |
| 计算分子量 | 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC026331 |
| 基因名称 | ITPKA |
| Gene ID (NCBI) | 3706 |
| RRID | AB_2129841 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23677 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITPKA(Inositol-trisphosphate 3-kinase A) belongs to the inositol phosphokinase (IPK) family. It accumulates in dendritic spines and seems to be critically involved in synaptic plasticity. The protein possesses two functional activities. In neurons, ITPKA is targeted to F-actin via an N-terminal actin binding domain (amino acids 1-66) and is relevant for long term potentiation and spatial learning(PMID:20022963).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPKA antibody 14270-1-AP | Download protocol |
| IHC protocol for ITPKA antibody 14270-1-AP | Download protocol |
| IF protocol for ITPKA antibody 14270-1-AP | Download protocol |
| IP protocol for ITPKA antibody 14270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7. | ||
J Mol Cell Biol Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum. | ||
Int J Cancer Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells.
| ||
Neuroscience Sex-specific Regulation of Spine Density and Synaptic Proteins by G-protein-coupled Estrogen Receptor (GPER)1 in Developing Hippocampus. | ||
Cytoskeleton (Hoboken) The catalytic domain of inositol-1,4,5-trisphosphate 3-kinase-A (ITPKA) contributes to ITPKA-induced modulation of F-actin. | ||
