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ITPKA Polyclonal antibody
ITPKA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3200 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14270-1-AP targets ITPKA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | ITPKA fusion protein Ag5588 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | inositol 1,4,5-trisphosphate 3-kinase A |
Synonyms | IP3K A, IP3 3-kinase A, InsP 3-kinase A, Inositol-trisphosphate 3-kinase A, Inositol 1,4,5-trisphosphate 3-kinase A |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC026331 |
Gene Symbol | ITPKA |
Gene ID (NCBI) | 3706 |
RRID | AB_2129841 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P23677 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ITPKA antibody 14270-1-AP | Download protocol |
IHC protocol for ITPKA antibody 14270-1-AP | Download protocol |
IF protocol for ITPKA antibody 14270-1-AP | Download protocol |
IP protocol for ITPKA antibody 14270-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neuron Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7. | ||
J Mol Cell Biol Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum. | ||
Int J Cancer Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells.
| ||
Neuroscience Sex-specific Regulation of Spine Density and Synaptic Proteins by G-protein-coupled Estrogen Receptor (GPER)1 in Developing Hippocampus. | ||
Cytoskeleton (Hoboken) The catalytic domain of inositol-1,4,5-trisphosphate 3-kinase-A (ITPKA) contributes to ITPKA-induced modulation of F-actin. | ||